腺病毒介导siCD44实验性治疗人CNE-2L2鼻咽癌
网页方法 以高表达CD44的人鼻咽癌细胞CNE-2L2为研究对象, 用软琼脂集落形成实验和细胞接种裸鼠后成瘤性生长及肿瘤肺转移检测细胞恶性生物学行为。构建Ad5-siCD44, 用293细胞包装产生病毒。接种CNE-2L2细胞于裸鼠皮下, 待肿瘤长到50～100 mm3大小, 瘤体内注 …
Inhibition of malignant activities of nasopharyngeal carcinoma …
网页Since CD44 was found to be significantly inhibited in the human nasopharyngeal carcinoma cell line CNE-2L2 with profound reduction of malignant activities caused by inhibition of alpha-mannosidase Man2c1 gene expression and CD44 has been observed to be involved in many tumor-supporting functions, we studied the association of CD44 expression ...
[Experimental siCD44-targeted therapy of human nasopharyngeal …
网页Objective: To explore the possibility of treating solid tumor with siCD44. Methods: Human nasopharyngeal carcinoma cell CNE-2L2 with high expression of CD44 was used in this study. The malignant activities of cells were examined by colony formation test, tumorigenesis, and lung metastasis of the tumor in nude mice.
鼻咽癌细胞CNE-2L2的蛋白质组学分析：与恶性生物学行为相关的 …
网页基础医学与临床 ›› 2007, Vol. 27 ›› Issue (4): 364-371.. • 研究论文 • 上一篇 下一篇 . 鼻咽癌细胞CNE-2L2的蛋白质组学分析：与恶性生物学行为相关的蛋白质 周异群 时岩 鞠吉雨 田云 曲莉 陈双玲 向志光 蒋东东 刘音 朱立平
网页CNE-2L2 cells in nude mice caused inhibition of tumor growth. The data indicate a positive association of CD44 expression with the malignant activities of CNE-2L2 cells and suggest a possible therapeutic effect of direct introduction of siRNA to CD44 into tumors in some human solid tumors with high expression of CD44 gene. Introduction
[Growth inhibition of human nasopharyngeal carcinoma cell CNE …
网页Methods: Recombinant adeno-associated virus vector (rAAV) was used as a mediator to transfer an antisense or a sense fragment of 6A8 cDNA into CNE-2L2 cells. 6A8 alpha-mannosidase expression was detected by means of mAb 6A8a staining, alpha-mannosidase activity assay and ConA binding test.
Inhibition of 6A8 α-mannosidase gene expression resulted in …
网页2005年2月10日 · Tumor from subcutaneously inoculated CNE-2L2 cells into nude mice has a high potentiality of metastasizing to the lung. CNE-2L2 cell with reduction of 6A8 α-mannosidase gene expression (AS) was developed by means of transduction with a DNA antisense to 6A8 cDNA [17].
Experimental siCD44-targeted therapy of human ... - ResearchGate
网页2007年10月1日 · Intra-tumor injection of Ad5-siCD44 can exhibit the therapeutic effect on the tumor inoculated with CNE-2L2 cells with high expression of CD44 in nude mice.
Tumor suppressor function of - Wiley Online Library
网页2009年9月14日 · BCSC-1 is dramatically upregulated in CNE-2L2 human nasopharyngeal carcinoma cells with reduced malignancy (AS cells) and is proposed to be a candidate tumor suppressor gene. We therefore examined the effect of BCSC-1 expression on malignant behaviors of CNE-2L2 cells.
Inhibition of α-mannosidase - Wiley Online Library
网页2008年12月3日 · Man2c1 suppression caused a decrease in the number of lamellipodia in the human nasopharyngeal carcinoma cell line CNE-2L2 and an increase in the numbers of filopodia and lamellipodia in the human T-cell leukemia cell line Jurkat. The data suggest that Man2c1 suppression might also affect arrangement of the …
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