Generation of a conditional Flpo/FRT mouse model expressing
网页2020年3月3日 · In order to analyze the effects of increased microenvironmental TGFβ concentration in vivo, we developed a conditional transgenic mouse model (Flpo/Frt system) expressing bioactive TGFβ in...
011065 - FLPo-10 Strain Details - The Jackson Laboratory
网页011065 B6.Cg-Tg (Pgk1-flpo)10Sykr/J This FLP deleter strain is a suitable alternative to, and complement with the Cre-loxP system for <i>in vivo</i> genetic engineering and may be useful for generating FRT-FLP conditional mutations.
Generation of a DAT-P2A-Flpo mouse line for intersectional …
网页2021年5月11日 · DAT-P2A-Flpo mice can be used together with Cre-expressing mouse lines to efficiently and selectively label dopaminergic subpopulations using Cre/Flp-dependent intersectional strategies. We demonstrate the utility of this approach by generating DAT-P2A-Flpo;NEX-Cre mice that specifically label Neurod6 -expressing dopamine neurons, which project ...
029591 - Slc32a1-2A-FlpO-D Strain Details - The Jackson Laboratory
网页FlpO expression and activity largely recapitulates the endogenous Slc32a1 gene - it is restricted to GABAergic neurons. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping related to GABAergic neuronal function.
A novel Nav1.8-FLPo driver mouse for intersectional genetics to …
网页2024年4月19日 · The Na v 1.8 Ires−FLPo mouse is an excellent model as it spatially restricts FLPo expression to a very small number of tissues, such as the DRG, TG, nodose ganglia (NG), and the conduction tissue of the heart. Temporal control over the expression and activation of the effector molecules depends on the induction of Cre recombination.
A single reporter mouse line for Vika, Flp, Dre, and Cre ... - Nature
网页2018年9月27日 · The multi-reporter mouse line for loci-of-recombination (X) (MuX) will be useful to characterize recombination characteristics of mice expressing either Cre, Flp, Dre or Vika recombinase and ...
High-Efficient FLPo Deleter Mice in C57BL/6J Background
网页2009年11月26日 · The new FLPo deleter mice largely facilitated our process of generating the GHR (growth hormone receptor) conditional knockout mice. The superior recombination efficacy of this FLPo deleter mouse line will be beneficial to the scientific community and particularly valuable for conditional gene manipulation in the …
The Cre-lox and FLP-FRT systems - The Jackson Laboratory
网页The FLP-FRT system is similar to the C re-lox system and is becoming more frequently used in mouse-based research. It involves using flippase (FLP) recombinase, derived from the yeast Saccharomyces cerevisiae (Sadowski 1995). FLP recognizes a pair of FLP recombinase target (FRT) sequences that flank a genomic region of interest. References.
Generation of a conditional Flpo/FRT mouse model expressing ... - PubMed
网页2020年3月3日 · In order to analyze the effects of increased microenvironmental TGFβ concentration in vivo, we developed a conditional transgenic mouse model (Flpo/Frt system) expressing bioactive TGFβ in fibroblasts, a cell population present in the microenvironment of almost all tissues.
网页we developed a conditional transgenic mouse model (Flpo/Frt system) expressing bioactive TGFβ in broblasts, a cell population present in the microenvironment of almost all tissues.