- [PDF]
cBot - Illumina
网页The cBot cluster generation system is the next generation of workflow improvements for Illumina sequencing. Novel innovations include pre-packaged reagents, a single manifold, advanced fluidics and thermal stage features, integrated sensors, remote
二代测序技术之illumina测序技术原理简介 - CSDN博客
网页2021年3月7日 · 第二代测序（Next-generation sequencing，NGS）又称为高通量测序，其开创性的引入了可逆终止末端，从而实现边合成边测序，在DNA复制过程中通过捕捉新添加的碱基所携带的特殊标记来确定DNA序列。
网页The cBot 2 Cluster Generation System provides many useful features, including positive tracking of samples and reagents throughout the cluster generation workflow, in addition to high-efficiency monoclonal cluster generation. The cBot 2 System integrates seamlessly into the complete Illumina sequencing workflow for accurate results with high
网页Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale sequencing. The innovative and flexible sequencing system enables a broad array of applications in genomics, transcriptomics, and epigenomics. Cluster Generation
NGS Workflow Steps | Illumina sequencing workflow
网页Libraries are sequenced on Illumina sequencing systems, designed to support a wide range of applications and throughputs. Data generated are then analyzed to gain insights. Learn the basics of each step and discover how to plan your NGS workflow.
网页Cluster Generation—For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment is then amplified into
网页Gentrain2 is the successor to Gentrain1, Illumina’s widely used cluster-ing algorithm for the BeadArray™ platform. While Gentrain1 has served effectively for some time, the increasing marker density per sample of Illumina microarrays has necessitated a revision of the algo-rithm to satisfy the escalating demands of these products. For typical
Cluster Generation - Illumina, Inc.
网页Cluster Generation. During cluster generation, single DNA molecules are bound to the surface of the flow cell, and then amplified to form clusters.
网页Registration and intensity extraction—For each cluster on the flow cell, the software records a cluster location and calculates an intensity value. Template generation—The software analyzes images from the first 5–7 cycles of a run to map the location of each cluster on a nonpatterned flow cell.
NovaSeq 6000 Reagent Kits - Illumina
网页NovaSeq 6000 Reagent Kits improvements include an enhanced reagent chemistry to streamline the workflow and accommodate more complex indexing strategies, an extended reagent shelf life of six months to provide flexibility in planning for future projects, and radio-frequency identification (RFID) encoded consumables to ensure reagent ...
- 某些结果已被删除