Golgi apparatus-synthesized sulfated glycosaminoglycans mediate ...
网页Using a genome-wide CRISPR-Cas9 screen to define factors critical for STING activation in cells, we identified proteins critical for biosynthesis of sulfated glycosaminoglycans (sGAGs) in the Golgi apparatus. Binding of sGAGs promoted STING polymerization through luminal, positively charged, polar residues.
网页Using a genome-wide CRISPR-Cas9 screen to define factors critical for STING activation in cells, we identified pro-teins critical for biosynthesis of sulfated glycosaminoglycans (sGAGs) in the Golgi apparatus. Binding of sGAGs promoted STING polymerization through luminal, positively charged, polar residues.
Sulphated glycosaminoglycans support an assortment of …
网页2017年3月16日 — We show that these structures are rich in sulphated glycosaminoglycans (sGAGs). Most of these (269 of 285 examined) appear to be topologically closed spheroids that we name ball-GAGs. Another class appears to burst to release flower- and star-like clusters which adhere to motile cilia.
Dual Action of Sulfated Hyaluronan on Angiogenic Processes in …
网页2019年12月2日 — This experimental and computational study revealed how sulfated GAG derivatives (sGAG) influence the interplay of vascular endothelial growth factor (VEGF)165 and its heparin-binding domain (HBD...
蒋争凡团队《Immunity》发文揭示硫酸化糖胺聚糖是STING活化的 …
网页2021年5月7日 — 该研究发现，高尔基体内合成的硫酸化糖胺聚糖（sGAGs）是STING共配体，介导STING在高尔基体的聚集和自激活。硫酸化糖胺聚糖分为四大类，其中硫酸角质素（keratan sulfate）是一种由二糖单元构成的直链线性聚合物，与STING的转运和激活密切相 …
Glycosaminoglycan Mimetic Precision Glycomacromolecules with …
网页Sulfated glycosaminoglycans (sGAGs) such as heparan sulfate (HS) are structurally diverse linear polysaccharides that are involved in many biological processes and have gained interest as antiviral compounds. Their recognition is driven by a complex orchestra of structural parameters that are still …
Cell-based semiquantitative assay for sulfated glycosaminoglycans ...
网页2015年10月1日 — The aim of this study was to develop a cell-based sGAG assay that facilitates the screening of chondrogenic compounds. The strategy of this assay is based on fluorescent sGAG staining with SO and DNA staining with 4’,6-diamidino-2 …
Identification of intracellular glycosaminoglycan-interacting proteins ...
网页2021年9月1日 — Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). Overall, 477 proteins were found interacting with at least one of four distinct sGAGs.
Sulfated hyaluronan alters fibronectin matrix assembly and …
网页2016年11月3日 — FN homes several binding sites for sulfated glycosaminoglycans (sGAG), such as heparin (Hep), which was previously shown to influence FN conformation and protein binding.
One-pot analysis of sulfated glycosaminoglycans
网页2017年12月5日 — A protocol for recovering and characterizing sulfated glycosaminoglycans (sGAGs) after metachromasia estimation is presented. The method involves decomplexing sGAG-DMMB complex, electrophoresis and HPLC analysis of disaccharide composition.
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