Having spent considerable time on preparation of ‘bottom’ agar plates for the agar layer assay by the plaque count method 1,2 and for high-titre bacterio-phage stock preparation on a large ...
It is critical that the digested fragments produce in-frame ... at 37 °C for 1 h and then plate them on doubly selective LB agar plates (100 μg/ml ampicillin and 35 μg/ml kanamycin).