Cells are typically fixed with 2% glutaraldehyde (e.g. in 100 mM Na-cacodylate or Na-phosphate buffer, pH 7.4) for 1-2 hours at RT post-fixed with 1% osmiumtetroxide for 1 h at RT dehydrated with ...

Fixation in cold ethanol and hand processing for IHC (Sainte-Marie G. A paraffin embedding technique for studies employing immunofluorescence. J Histochem Cytochem 10:250-256, 1962) Colitis models for ...