Figure 1: The generation of embryonic stem cells through in vitro ... each cell of the blastocyst and about 10 per primordial germ cell as these are first specified in the early postimplantation ...

Accumulating evidence indicates that epigenetic modifiers have key roles in genome-wide reprogramming and activation and repression of specific genes during mammalian germ-cell development.

such as the differing types of blood system cells. A second source is embryonic germ cells that are derived from the primordial reproductive tissues of aborted early fetuses. These are the cells ...

Knockout and knock-in mouse lines can be generated using gene targeting in embryonic stem cells with chimera production or CRISPR genome editing in mouse zygotes. For projects involving CRISPR genome ...