Polymerase chain reaction, or PCR, is a laboratory technique ... the temperature of the sample in automatic, programmed steps. Initially, the mixture is heated to denature, or separate, the ...

When the first thermal cyclers for the polymerase chain reaction came out in the 1980s ... PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single ...

In the polymerase chain reaction (PCR), for example, recognition is provided by sequence-specific hybridization of short oligonucleotides to a larger target, whereas signal amplification requires ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...