The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...

This method was named for its similarity to the technique known as a Southern blot. The first step in a northern blot is to denature, or separate, the RNA within the sample into single strands ...