The sections were immediately transferred to a cold room (4 °C), where 10 μl of matrix (sinapinic acid, 10 mg/ml in acetonitrile/0.05% trifluoroacetic acid 50:50) were deposited with a pipette ...
Stop the reaction by addition of 1.5 μl of 0.1% trifluoroacetic acid. 10| Fractionate the peptides by narrow-bore high-performance liquid chromatography. Subject the collected peptides to ...
Figure 5. Temperature dependence of the rate constant. Figure 6. Activation energy graph - Arrhenius plot. The reaction follows pseudo-first-order kinetics due to the fact that trifluoroacetic acid ...