传统的Western Blot,耗时长,手工操作多,重现性差(CV>35%)。遇到珍贵样本(蛋白量不足)通常会进行strip-reprobe(洗脱-重杂交)的方式进行蛋白质多靶点的分析。 然而,传统Western Blot进行strip-reprobe(洗脱-重杂交)时会面临: 洗脱次数不够,无法充分解离靶点 ...
A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue ...
Quantum Dot Corporation's (QDC) new western blot detection kits harness the high brightness, extreme stability and multiple colors of the Qdot Secondary Antibody Conjugates to quantitatively ...
The multiplex fluorescent detection and chemiluminescent imaging capabilities of the FluorChem Q provide a complete solution for quantitative Western blot imaging and analysis. The open platform ...
随后在小鼠血清中检测肝功能指标,同时对肝组织进行病理和透射电镜观察。采用CCK-8法检测L-O2细胞活性,采用qPCR、免疫组织化学和western blotting检测肝组织和L-O2细胞中DNA损伤诱导转录因子4 (DDIT4) ...
• 自动回收一抗。 • 只需加入样本和缓冲液即可,无复杂安装。 更高的信噪比。专利的流体技术可以实现更加充分的清洗,减少二抗在膜上的附着,降低曝光的背景值。 更好的重复性。自动化操作避免了手工操作的误差,使得批次间的结果更有可比性。