DMSO. Ligand 2 was prepared by dilution with the same buffer used for proteins. Aliquots (2 μL) of the ligand solution at 20× the binding-site concentration were added by means of a 100 μL rotating ...
Samples are divided and stored as multiple small aliquots to ensure the maximum possible use. A DNA bank has been extracted from blood, cell line, and mouthwash samples at various timepoints. In ...
Pipet the emulsion into the wells of a PCR plate as 10 aliquots of 50 μl. Pipet 50 μl of the aqueous phase (Step 3) into a well as a nonemulsified control. Overlay the emulsified and ...
Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...
Sickness-induced sleep is a behavior conserved across species that promotes recovery from illness, yet the underlying mechanisms are poorly understood. Here, we show that interleukin-6-like cytokine ...
The vast majority of bacteria live in sessile biofilms that colonize the channels, pores and crevices of confined structures. Flow in these structures carries the nutrients necessary for growth, but ...