To define the effects of exon 3 deletion on Mecp2 expression, we compared RNA and protein from mutant and control brains. Northern-blot analysis detected a slightly smaller Mecp2 transcript at a ...
10. Tighten caps, shake well and leave in the fume hood for 1-2 min for phase separation. 11. Using a pasteur pipette, carefully transfer the upper hexane phase into small 2 ml glass vials; this ...