The schematic diagram [Figure (b)] shows that the Stokes beam is modulated with an electro-optic modulator (EOM, M350-160 KD*P, Conoptics) at a couple of MHz by using a square-wave function-generator ...

Stimulated Emission Depletion (STED) microscopy is a super-resolution imaging technique that overcomes the diffraction limit of conventional optical microscopy. It enables the visualization of ...