Amplicons were recovered from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) according to manufacturer’s instructions and quantified using a ...
Samples (mouse zygotes, embryos or ESCs with zona pellucida removed) were transferred into 0.2mL conventional, non-low-binding PCR tube (Axygen). The samples were resuspended by 60μL washing buffer ...