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来自MSN
17 天
提取RNA时,如何摆脱RNA酶污染?
在试剂方面,要使用经处理的无 RNA 酶水来配制溶液 ... 如焦碳酸二乙酯(DEPC)。不过需要注意的是,DEPC 本身有一定毒性,使用后要保证其完全 ...
来自MSN
20 天
如何在提取RNA时,有效避免RNA酶污染?
建议采用已经过特殊处理去除了RNase活性的水作为溶剂基础 ... 可以考虑添加适量的RNase抑制剂如DEPC,但需注意这种物质具有一定毒性,在最终产品 ...
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