Offering a bridge between traditional 2D techniques and animal models, 3D cell culture is providing a wealth ... that a lot of the genes that promoted growth in a 3D-specific context ended up ...

Using appropriate methods (Schedule 1; Animals ... cells at 200g for 5 min at 15–25 °C and resuspend the pellet in 10 ml MEF medium supplemented with 4 ng/ml bFGF. To prepare the appropriate ...

Cell culture media are formulated to provide the necessary nutrients, growth factors, and pH for cell growth ... and mechanisms of action before proceeding to animal studies and clinical trials. Cell ...