The study, led by Edith Alejandra Carreño and her colleagues at the Oswaldo Cruz Foundation in Brazil, reveals a series of pitfalls in how the MTT assay is commonly used. The researchers are now ...
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Then, 20 µL of MTT solution (5 mg/mL) was added to each well and incubated for 1.5 h at 37 ... The numbers of apoptotic and necrotic cells were measured using a FACS Calibur flow cytometer (Beckton ...
For MTT assay 3,000 cells were seeded per well in a 96-well plate ... The stained cells were filtered through a 400-mesh sieve and analyzed using a BD Accuri C6 flow cytometer and the FlowJo v10.6 ...
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