Cell competition in epithelial tissue eliminates transformed cells expressing activated oncoproteins to maintain epithelial homeostasis. Although the process is now understood to be of mechanochemical ...
Cells were then harvested, fixed in 70% of ethanol in Phosphate-Buffered Saline (PBS) for 24 h at −20°C and incubated with 40 μg/mL RNase A and 20 μg/mL Propidium Iodide (both from Sigma-Aldrich) for ...
For analysis of barrier dysfunction, mice were orally administered 0.6 mg/g body weight of fluorescein isothiocyanate (FITC)-dextran (4 kDa, Sigma-Aldrich) in phosphate-buffered saline (PBS), and ...
4 Briefly, 96-well plates were coated with 100 µL of the recombinant protein at a concentration of 2.5 µg/mL in phosphate buffered saline (PBS) for 2 hours at 25°C. CSF and serum samples were diluted ...
Fresh specimens of murine pancreas were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS, pH 7.4). Tissues were embedded in paraffin ... l phenylmethanesulfonyl fluoride and a cocktail ...
The "Sigma Sigma Boy" trend, featuring a song by Russian artists Betsy and Masha Yankovskaya, has taken social media by storm. The trend celebrates the "Sigma male" archetype – independent ...
The birds in the Dex and Dex+V groups were injected with DEX (D4092, Sigma-Aldrich, MO, USA) via the intracoelomic route (dose = 0.9 mg/kg ... Single staining was performed using the antibodies ...
This is an important study using a combination of optogenetics and calcium imaging to provide insight into the function of the cholinergic input to the prelimbic cortex in probabilistic spatial ...