The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

This method was named for its similarity to the technique known as a Southern blot. The first step in a northern blot is to denature, or separate, the RNA within the sample into single strands ...

This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...