Centrifuge the cells at 200g for 5 min at 15–25 °C and resuspend the pellet in 10 ml MEF medium supplemented with 4 ng/ml bFGF. To prepare the appropriate culture vessels for plating the ...

The standard 2-D cell-culture systems used today, which grow PSCs as monolayers on flat, rigid plastic or glass surfaces, can't economically produce enough cells for many important therapies.

Cell culture is a fundamental technique in biotechnology and life sciences that involves growing and maintaining living cells outside their natural environment, typically in a controlled laboratory ...

Human induced pluripotent stem cells (hiPSCs) are considered a promising tool in medicine, with the potential to unlock ...

Huch’s pancreatic organoids are now a promising avenue for disease modeling and generating precision medicines to treat ...

However, high raw material costs impede financial viability. One area that could be improved is stem cell culture media, which are often expensive and can contain components unsuitable for food ...

During the development of the olfactory epithelium (OE), olfactory sensory neurons (OSNs) express only one member of the odorant receptor (OR) gene family, and O ...

Tumor cells circulating in the blood are the "germ cells" of breast cancer metastases. They are very rare and could not be propagated in the culture dish until now, which made research into therapy ...

Tumor cells circulating in the blood are the “germ cells” of breast cancer metastases. They are very rare and could not be propagated in the culture dish until now, which made research into therapy ...