or streptavidin-fluorophore conjugates rather than antibodies to detect biotin tags. With its specificity, clarity and relative rapidity of detection, FISH remains the technique of choice for ...
We set out to elucidate its biology by identifying and characterising citrullinated antigen-specific B cells in peripheral blood of patients with RA. Methods Differentially labelled streptavidin and ...
School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong ...
Unlabeled antibodies and biotin-conjugated antibodies were incubated with secondary anti-mouse antibodies conjugated with Texas Red and streptavidin-APC conjugates, respectively, at 4°C for 45 min in ...
2 (1:400 dilution; AbD Serotec, UK; MCA771GA)) in 1% BSA/PBS overnight at 4°C. Detection was carried out with biotinylated goat anti-rabbit secondary antibodies (1:200 dilution; Dakocytomation), ...
Back Pain Unit, King’s Mill Hospital, Sutton in Ashfield, Notts NG17 4JL, UK Dr D A Walsh, Academic Rheumatology, University of Nottingham Clinical Sciences Building, City Hospital, Hucknall Road, ...