Frozen sections were immunohistochemically stained with monoclonal antibody to chick Pax6 (amino acids 1–223). To verify antibody specificity, western blot analysis was carried out using cell lysates ...
For example, hiking up the contrast of a western blot image to decrease the appearance ... reviewers and ultimately readers. When modifications are unavoidable, authors should provide a clear ...
The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP antisera. The GFP fluorescence (F) was quantified by fluorescence spectroscopy of whole-cell lysates.
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A single amino acid change in a neuronal ion channel called KCNQ2 blocks ion flow, prevents protein localization on axons, and results in severe epilepsy and slowed neurological development.