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afzhan
17 天
LS 174T细胞 人结直肠腺癌细胞
1. 吸出原培养瓶中的培养基,PBS 缓冲液润洗细胞两次,加 2-3 ml 0.25% 胰_酶进行消化细胞(注意把握消化时间,通常控制在 1-2min) 2. 镜下观察消化情况,在细胞边缘缩小,贴壁松动时(不建议消化到细胞漂浮)去掉胰_酶,加 6~8ml 培养基,轻轻吹打细胞层 ...
搜狐
29 天
王广基院士团队全面阐述间充质干细胞药代动力学特征与临床转化挑战
在过去几十年中,间充质干细胞(Mesenchymal stem/stromal cells,MSCs)疗法实现了显著的飞跃与突破。尽管该疗法在攻克难治性及重症疾病方面展现出了极为广阔的应用前景,并已在临床实践与商业领域取得了初步而可喜的成就;然而,MSCs疗法在临床转化的过程中 ...
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