Reversed-phase chromatography - Wikipedia
网页Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [1][2][3] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the reversed ...
反相高效液相色谱 - 百度百科
网页rp-hplc是当今液相色谱的最主要的分离模式，几乎可用于所有能溶于极性或弱极性溶剂中的有机物的分离。 反相色谱法适于分离非极性、极性或离子型化合物，大部分的分析任务皆由反相色谱法完成。
Reversed-Phase High-Performance Liquid Chromatography
网页Reversed-phase high-performance liquid chromatography (RP-HPLC) involves the separation of molecules on the basis of hydrophobicity. The separation depends on the hydrophobic binding of the solute molecule from the mobile phase to the immobilized hydrophobic ligands attached to the stationary phase, i.e., the sorbent.
12.5: High-Performance Liquid Chromatography - Chemistry …
网页2021年11月13日 · In reversed-phase chromatography, which is the more common form of HPLC, the stationary phase is nonpolar and the mobile phase is polar. The most common nonpolar stationary phases use an organochlorosilane where the R group is an n -octyl (C 8 ) or n -octyldecyl (C 18 ) hydrocarbon chain.
Reversed Phase HPLC - an overview | ScienceDirect Topics
网页Reversed-phase HPLC refers to the most commonly used type of HPLC for analyzing organic compounds. It involves selecting a stationary phase and corresponding column based on the chemistry, physical characteristics, and dimensions of the column, as well as the nature of the analytes and the sample matrix.
Reverse Phase Liquid Chromatography - ScienceDirect Topics
网页Reversed-Phase Liquid Chromatography (RPLC) is one of the High Pressure Liquid Chromatography or “HPLC” approaches, which are now for commercial reasons often alluded to as High Performance Liquid Chromatography. RPLC is especially useful in the separation and purification of proteins.
Reversed-Phase Chromatography - an overview - ScienceDirect
网页2011年1月6日 · Reversed-phase chromatography is the most common HPLC separation technique and is used for separating compounds that have hydrophobic moieties and do not have a dominant polar character (although polarity of a compound does not exclude the use of RP-HPLC). From: Essentials in Modern HPLC Separations, 2013.
How Reversed-Phase Liquid Chromatography Works
网页2013年8月1日 · This tutorial on reversed-phase LC explains the role of solvent, chain conformation, solute position, and retention dynamics. The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation.
Chapter 8 Reversed Phase Liquid Chromatography - Wiley …
网页2015年11月25日 · Reversed phase liquid chromatography (RPLC) is a mode of high-performance liquid chromatography (HPLC) that employs a nonpolar stationary phase (most frequently a hydrocarbon chain chemically bonded to porous silica particles) and a polar mobile phase constituted by water and at least a water-miscible organic solvent, which performs as a modifier.
网页2013年8月29日 · Reverse Phase. How proteins separate on reverse-phase HPLC. RP chromatography separates proteins through the interaction of the “hydrophobic foot” of the protein with a non-polar surface of the particle. RP …