Hammerhead ribozyme - Wikipedia
The hammerhead ribozyme is an RNA motif that catalyzes reversible cleavage and ligation reactions at a specific site within an RNA molecule. It is one of several catalytic RNAs (ribozymes) known to occur in nature.
Self-cleaving guide RNAs enable pharmacological selection of …
2022年11月30日 · The scgRNA consists of a gRNA flanked by two self-cleaving ribozymes: a hammerhead (HH) ribozyme located 5′ to the gRNA and a hepatitis delta virus (HDV) ribozyme located 3′ to the gRNA.
The Hammerhead Ribozyme: Structure, Catalysis and Gene …
The hammerhead ribozyme has long been considered a prototype for understanding RNA catalysis, but discrepancies between the earlier crystal structures of a minimal hammerhead self-cleaving motif and various biochemical investigations frustrated attempt to understand hammerhead ribozyme catalysis in terms of structure.
Hammerhead Ribozyme - an overview | ScienceDirect Topics
A Hammerhead Ribozyme is a type of ribozyme with a conserved catalytic core that can cleave RNA at specific triplets. It requires divalent metal ions like magnesium for its catalytic activity, although its application in vivo conditions is uncertain due to high magnesium requirements.
Scott Lab: Hammerhead Ribozyme - UCSC
The hammerhead ribozyme is a small catalytic RNA that was discovered in 1986, and William Scott began structural studies on it in 1987 as a graduate student with Sung-Hou Kim at Berkeley, and continued to do so with Aaron Klug (MRC, Cambridge) as a postdoc.
The ubiquitous hammerhead ribozyme - PMC - National Center …
The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self-scission under physiological conditions.
The Hammerhead Ribozyme: A Long History for a Short RNA
2017年1月4日 · The hammerhead ribozyme has been regarded as a biological oddity typical of small circular RNA pathogens of plants. More recently, numerous and new variations of this ribozyme have been found to inhabit the genomes of organisms from all life kingdoms, although their precise biological functions are not yet well understood.
The hammerhead self-cleaving motif as a precursor to complex
Here we show that neutral networks of two naturally occurring, seemingly unrelated endonucleolytic ribozymes, the hammerhead (HH) and hairpin (HP), intersect. Sequences at the intersection of these networks exhibit catalytic functions corresponding to both ribozymes by potentially populating both catalytic folds and enable a smooth crossover ...
Cis-Acting Ribozymes for the Production of RNA In Vitro
2012年1月1日 · This chapter describes the use of cis-acting ribozymes, 5′-end hammerhead (HH) and 3′-end hepatitis delta virus (HDV), for direct transcriptional processing to yield target RNAs with precisely defined ends. The method is focused on the use of the pRZ and p2RZ plasmids that are designed to simplify the production of such dual ribozyme templates.
Highly Active Low Magnesium Hammerhead Ribozyme
2009年1月4日 · Hammerhead (HH) ribozymes can be used for highly specific inhibition of gene expression through the degradation of target mRNA. In vitro experiments with minimal HH domains demonstrated that the efficiency of catalysis is highly dependent on concentration of magnesium ions.