Primary cell culture - Wikipedia
网页Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.
Primary Cell Culture Basics - MilliporeSigma
网页Primary cell culture is increasingly being used as a major tool in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging, signaling studies), the effects of drugs and toxic compounds on the cells and mutagenesis and carcinogenesis.
Primary Cell Culture Guide - ATCC
网页Primary cell cultures are commonly used as in vitro tools for preclinical and investigative biological research, such as studies of inter- and intracellular communication, developmental biology, and elucidation of disease mechanisms, such as …
A Beginner’s Guide to Cell Culture: Practical Advice for Preventing ...
网页2023年2月21日 · Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or chemicals.
网页ATCC has provided a solution to help investigators overcome the high cost and inconsistency found in routine primary cell culture with the development of ATCC Primary Cell Solutions, a standardized cell culture system that includes high quality cells, media, supplements, reagents, and protocols.
Primary Cell Culture - an overview | ScienceDirect Topics
网页Primary cell culture is defined as the ex vivo culture of cells freshly obtained from a tissue. The main advantages of primary cells are their faithful transcriptomic and proteomic profile and closer proximity to physiological function and response.
An Introduction to Primary Cell Culture - Lonza
网页Primary cells resemble the tissue from which they were acquired and have low levels of genetic mutations but cannot be passaged indefinitely. Despite their limited lifespan, primary cells capture the complexity of living tissues, crucial for personalized medicine.
Introduction to Cell Culture | Thermo Fisher Scientific - US
网页Primary Culture. Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate (i.e., reach confluence).
Cell Culture - PMC - National Center for Biotechnology Information
网页2017年4月7日 · Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells. Primary cells , such as fibroblasts obtained from skin biopsies and hepatocytes isolated from liver …
网页2015年3月9日 · Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.