Viroplasms: Assembly and Functions of Rotavirus Replication …
Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers of viroplasm ...
Rotavirus infection | Nature Reviews Disease Primers
2017年11月9日 · Reinfections with rotavirus are common throughout life, although the disease severity is reduced with repeat infections. The immune correlates of protection against rotavirus reinfection and...
The rotavirus replication cycle [From: Crawford, SE et al, Rotavirus …
Internalization of RV particles occurs by receptor-mediated endocytosis and is followed by removal of the outer layer in the endosome, resulting in the release of DLPs into the cytoplasm. The DLPs...
Rotaviruses - ScienceDirect
2014年9月22日 · The rotavirus replication cycle. The rotavirus triple layered particles (TLPs) first attach to sialo-glycans (or histo-blood group antigens) on the host cell surface, followed by interactions with other cellular receptors, including integrins and Hsc70. Virus is then internalized by receptor-mediated endocytosis.
In this paper, we review various aspects of the rotavirus replication cycle, focusing on the role of Ca2+ in entry and assembly of the new particles, and provide new elements on Ca2+ homeostasis changes during infection.
Rotavirus-Classification, Structure composition and Properties ...
2017年5月21日 · Replication: Occurs in cytoplasm of infected cell. Viral encoded toxin: early profuse, secretory diarrhea is caused by enterotoxin, NSP4. The acute infection and diarrhoea normally resolves within 7 days in immunocompetent hosts. Acute Gastroenteritis, severe in case of infants aged 6-24 months.
Molecular biology of rotavirus entry and replication - PubMed
2009年12月16日 · Remarkable progress in the understanding of the rotavirus cycle has been made in the last 10 years. The knowledge of viral replication thus far acquired is based on structural studies, the expression and coexpression of individual viral proteins, silencing of individual genes by siRNAs, and the effects that these manipulations have on the ...
Viroplasms: Assembly and Functions of Rotavirus Replication ... - PubMed
2021年7月12日 · Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers of viroplasm formation. The structures (as far as is known) and functio …
Rotavirus Replication and Reverse Genetics - ScienceDirect
2016年1月1日 · In the RV replication cycle, the outer capsid protein layer of virions is lost, producing DLPs that express low levels of viral (+)RNA at early times of infection. Translation of the (+)RNAs produces proteins that promote the formation of viroplasms, alter the host translational apparatus, and inhibit the development of an antiviral state.
Characterization of the rotavirus assembly pathway
2023年4月12日 · We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular lamellae. Our findings demonstrate that the viral polymerase VP1 recruits viral genomes during particle assembly, as revealed by infecting with a conditionally lethal mutant.