Adaptation of single virus fusion assay to study fusion of dengue virus ...
2024年2月8日 · This assay observes fluorescently-labeled DENV virus-like particles (VLPs) fusing to target liposomes, tethered to a polymer surface in a microfluidic device. VLPs are bound to the target liposomes using DNA-lipid conjugates as surrogate viral receptors.
Structure of Dengue Virus: Implications for Flavivirus …
In dengue virus, the E dimers are so closely packed that the viral membrane remains inaccessible and fusion is impossible at neutral pH. We, therefore, hypothesize that fusion is initiated by the low pH-induced conformational change, creating the predicted T = 3 structure (see above).
Dengue Virus Ensures Its Fusion in Late Endosomes Using …
We propose that anionic lipid-dependence of DEN fusion machinery protects it against premature irreversible restructuring and inactivation and ensures viral fusion in late endosomes, where the virus encounters anionic lipids for the first time during entry.
Membrane fusion by dengue virus: The first step - ScienceDirect
Among the structural proteins the viral genome encodes, perhaps the fundamental one, is the membrane fusion protein (MFP), the class II membrane fusion envelope E protein, which is in charge for the viral and cellular membrane fusion.
Structure of the dengue virus envelope protein after membrane fusion
2004年1月22日 · Dengue virus enters a host cell when the viral envelope glycoprotein, E, binds to a receptor and responds by conformational rearrangement to the reduced pH of an endosome. The conformational...
Dengue virus ensures its fusion in late endosomes using
2010年10月7日 · Here we show that DEN utilizes bis(monoacylglycero)phosphate, a lipid specific to late endosomes, as a co-factor for its endosomal acidification-dependent fusion machinery. Effective virus fusion to plasma- and intracellular- membranes, as well as to protein-free liposomes, requires the target membrane to contain anionic lipids such as bis ...
On the role of surrounding regions in the fusion peptide in dengue ...
We propose a 2-step mechanism for the interaction of the dengue virus fusion peptide with the host membrane, where the N-terminal sequence docks electrostatically on the headgroups and then the C-terminal interacts via hydrophobic forces in the acyl chains.
Dengue Virus Ensures Its Fusion in Late Endosomes Using ... - PLOS
2010年10月7日 · As with many other viruses, dengue virus enters cells by fusion between the viral membrane and the membrane of intracellular vesicles (endosomes). In this work we explored the fusion stage of dengue virus entry in different experimental systems ranging from virus fusion to artificial lipid membranes to fusion inside the cells.
Antibody-Dependent Enhancement of Dengue Virus Infection in …
2016年7月6日 · We discovered that, in primary human macrophages, antibody-mediated cell entry of DENV enhances the fusion potential of the virus. No enhanced binding and entry was seen. Also, we observed that...
Dengue virus IgG and neutralizing antibody titers measured with ...
4 天之前 · The standard dengue virus (DENV) neutralization assay inconsistently predicts dengue protection. ... Mature virions lack prM, resulting in only intermittent exposure of the fusion loop and a dimer ...