Cell division - WormBook
The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the syncytial gonad makes it possible to use RNA interference (RNAi) to generate oocytes whose cytoplasm is reproducibly ( > 95%) depleted of targeted essential gene products.
Cell division - WormBook - NCBI Bookshelf - National Center for ...
2006年1月19日 · The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the syncytial gonad makes it possible to use RNA interference (RNAi) to generate oocytes whose cytoplasm is reproducibly (>95%) depleted of targeted essential gene products.
Employing the One-Cell C. elegans Embryo to Study Cell Division ...
In this chapter, we describe methods for studying cell division processes in the one-cell C. elegans embryo. We detail approaches for engineering essential cell division genes, visualizing their products, and conducting quantitative imaging based assays to define phenotypes.
Computable early Caenorhabditis elegans embryo with a phase
2022年1月14日 · Simulations with variable parameters indicated that a delicate program of cell division timing, cell division orientation, and cell-cell attraction matrix is crucial for precise and robust morphogenesis, in particular for establishing the correct cell-cell contact map, which serves as the physical basis for many biological regulations.
Mitotic Cell Division in Caenorhabditis elegans
2019年1月8日 · Mitotic cell divisions increase cell number while faithfully distributing the replicated genome at each division. The Caenorhabditis elegans embryo is a powerful model for eukaryotic cell division. Nearly all of the genes that regulate cell division in C. elegans are conserved across metazoan species, including humans.
C. elegans cells in different stages of cell division. They characterize structural features of centrioles during their assembly and of a disordered matrix that nucleates and organizes mitotic spindle microtubules. C. elegans Embryos Dissociated Embryonic Cells Fluorescence-Targeted Preparation and Cryo-ElectronTomography Mother Centriole
Molecular architectures of centrosomes in C. elegans embryos …
5 天之前 · To provide insight into the structural evolution of centrioles and the PCM throughout the cell cycle, here we use cryogenic electron tomography (cryo-ET) on dividing C. elegans embryonic cells. In contrast to traditional cellular EM employed in most ultrastructural studies of centrosomes to date, this method allows the preservation of fine structural detail. 58 In particular, the fast cooling ...
Molecular architectures of centrosomes in C. elegans ... - Cell Press
Tollervey et al. use cryo-electron tomography to capture 3D snapshots of centrosomes at molecular resolution in their near-native state inside embryonic C. elegans cells in different stages of cell division. They characterize structural features of centrioles during their assembly and of a disordered matrix that nucleates and organizes mitotic spindle microtubules.
Employing the one-cell C. elegans embryo to study cell division ...
The one-cell Caenorhabditis elegans embryo offers many advantages for mechanistic analysis of cell division processes. Conservation of key genes and pathways involved in cell division makes findings in C. elegans broadly relevant.
Embryo Introduction - Wormatlas
2017年11月1日 · After fertilization, the single cell embryo begins a series of highly stereotyped cell divisions. During this phase, all embryonic cells look similar in their cytoplasmic structures, and many cells also begin to make short distance migrations away from the their sister cells, through a process that has been termed “global cell sorting”.