In Vitro Culturing and Screening of Candida albicans Biofilms
网页Candida albicans is a normal member of the human microbiota that asymptomatically colonizes healthy individuals, however it is also an opportunistic pathogen that can cause severe infections, especially in immunocompromised individuals.
Automated quantification of Candida albicans biofilm-related …
网页2020年10月9日 · Here, we designed an informatics pipeline for quantifying filamentation, adhesion, and invasion of Candida species on solid agar media and utilized this approach to determine the importance of...
Candida albicans biofilms: development, regulation, and …
网页C. albicans biofilm formation begins with the adherence of round yeast cells to a solid surface (in the laboratory, a small silicone disc, the material of common intravascular catheters, or a polystyrene microtiter plate, are often used).
Screening of Candida albicans GRACE library revealed a unique …
网页2019年6月24日 · Candida albicans biofilm formation is governed by a regulatory circuit comprising nine transcription factors which control a network of target genes.
A 96 Well Microtiter Plate-based Method for Monitoring …
网页2010年10月21日 · Here we describe a simple, rapid, economical and highly reproducible 96 well microtiter plate model for the formation of Candida biofilms coupled with a colorimetric method that measures the metabolic activities of cells within the biofilm using XTT.
A simple and reproducible 96-well plate-based method for the
网页2008年8月28日 · Introduction. Fungal infections caused by yeasts and moulds represent an escalating problem in health care, as advances in modern medicine prolong the lives of not only severely ill patients,...
A Simple 96-Well Plate-Based Method for Development of Candida …
网页2022年8月26日 · 1 Introduction. In their natural ecosystems, most microbes exist as biofilms [1]. Biofilm is defined as surface-associated, highly structured community of microorganisms that are enclosed within a self-produced protective extracellular matrix (ECM) [2].
A 96 well microtiter plate-based method for monitoring ... - PubMed
网页2010年10月21日 · DOI: 10.3791/2287. Abstract. Candida albicans remains the most frequent cause of fungal infections in an expanding population of compromised patients and candidiasis is now the third most common infection in US hospitals.
A 384-Well Microtiter Plate Model for Candida Biofilm ... - PubMed
网页Here we describe the development of a novel, simple, fast, economical, and highly reproducible 384-well microtiter plate model for the formation of both Candida albicans and Candida auris biofilms and its application in high-throughput screening (HTS) techniques. Keywords: Antifungals; Biofilms; Candida; Candidiasis; High throughput screening.
网页Candida albicans is a normal member of the human microbiota that asymp-tomatically colonizes healthy individuals, however it is also an opportunistic pathogen that can cause severe infections, especially in immunocompromised individuals.