Haemophilus Serotyping: Introduction, Principle, Test Requirements
2023年2月19日 · Interaction between the antibody (Ab) and particulate antigen results in visible clumping called agglutination. In brief, the serological test is based on the fact that antibodies in serum, produced in response to exposure to bacterial antigens, will agglutinate with bacteria carrying homologous antigens. Test Requirements for Haemophilus ...
Comparison of methods for serotyping isolates of Haemophilus influenzae ...
This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and ...
L27 - Medical Microbiology Laboratory (Haemophilus spp.)
2018年9月19日 · This document discusses three medically important species of Haemophilus: H. influenzae, H. ducreyi, and H. aegyptius. H. influenzae is an obligate human parasite that can cause both acute and chronic infections, including meningitis, pneumonia, and sepsis. It has six serotypes defined by capsular antigens.
Direct Real-Time PCR for the Detection and Serotyping of Haemophilus …
To monitor the burden and changes in Haemophilus influenzae (Hi) disease, direct real-time PCR (drt-PCR) assays have been developed for Hi detection in monoplex form and its six serotypes in triplex form, directly from cerebrospinal fluid (CSF) specimens.
Comparison of methods for serotyping isolates of Haemophilus
This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticaps …
Laboratory diagnosis of Haemophilus ducreyi infection
1995年12月1日 · Various non-culture tests for detection of H. ducreyi were developed. As for other diseases, the polymerase chain reaction (PCR) test is proving its usefulness for diagnosing chancroid. However, sample handling should be optimized and criteria allowing the classification of culture negative/PCR positive patients should be established.
The laboratory diagnosis of Haemophilus ducreyi - PMC
When investigating GUD that is believed to be due to H ducreyi, include herpes simplex culture and syphilis testing (direct fluorescence antibody [DFA] or serum for rapid plasma reagin testing). For suspect cases of chancroid, testing for herpes simplex virus and syphilis should also be performed (Table 1).
Direct Examination | Haemophilus - Manual of Clinical Microbiology
2023年9月13日 · Identification of Haemophilus influenzae serotypes by standard slide agglutination serotyping and PCR‐based capsule typing. J Clin Microbiol 41:393–396. Google Scholar
A comparison between methods of identification and serotyping …
Seven methods of serotyping of Haemophilus influenzae were evaluated. Comparing slide agglutination, staphylococcal coagglutination, latex agglutination, counterimmunoelectrophoresis, immunofluorescence, capsular swelling, and cultivation on antiserum agar the commercial coagglutination test was mos …
Haemophilus influenzae: Characteristics, Pathogenesis and Lab …
Slide agglutination test. Apply one drop of normal saline on a slide and make a homogenous suspension with a single suspected colony of H. influenzae. Add one drop of specific antiserum and mix thoroughly.