Structure of the human chromosome interaction network - PMC
New Hi-C technologies have revealed that chromosomes have a complex network of spatial contacts in the cell nucleus of higher organisms, whose organisation is only partially understood. Here, we investigate the structure of such a network in human GM12878 cells, to derive a large scale picture of nuclear architecture.
Structure and Dynamics of Interphase Chromosomes - PLOS
2008年8月22日 · Each chromosome consists of a single continuous DNA double-helix and associated proteins that organize locally in the form of a chromatin fiber. During cell division (mitosis) chromosomes adopt a compact form that is suitable for transport. During periods of normal cell activity (interphase), they decondense inside the cell nucleus.
Chromosomes at Work: Organization of Chromosome Territories …
Chromosome organization in CTs during interphase is now widely accepted in the scientific community. Currently, two main techniques are used to study chromosome territory organization: fluorescence in situ hybridization (FISH) and chromatin conformation capture.
Principles of Chromosome Architecture Revealed by Hi-C - PMC
Chromosomes are folded and compacted within interphase nuclei, but the molecular basis of this folding is poorly understood. Chromosome conformation capture methods, such as Hi-C, combine chemical cross-linking of chromatin followed by fragmentation, DNA ligation, and high-throughput DNA sequencing to detect neighboring loci genome-wide.
Interchromosomal interactions: A genomic love story of kissing chromosomes
More recently, it has become clear that not only intrachromosomal interactions, but also interchromosomal interactions, a less studied feature of chromosomes, are required for proper physiological gene-expression programs. Here, we review recent studies with emerging insights into where and why cross-chromosomal communication is relevant.
Chromosome positioning in the interphase nucleus - Cell Press
2002年9月1日 · Metaphase chromosomes display a chromosome-specific series of alternating light- and dark-staining bands (R- and G-bands), corresponding to early- and late-replicating regions of the genome. During interphase, these regions are maintained and occupy distinct domains within a chromosome territory [10,14,15] .
Meiotic double-strand breaks at the interface of chromosome …
2003年11月1日 · Double-strand breaks (DSBs), programmed or γ-rays-induced, are found to promote four major events beyond recombination and accompanying synaptonemal complex formation: (1) juxtaposition of homologs from long-distance interactions to close presynaptic coalignment at midleptotene; (2) structural destabilization of chromosomes at leptotene ...
Three-dimensional positioning and structure of chromosomes in …
2017年7月21日 · We show a three-dimensional (3D) image of a human prophase nucleus obtained by serial block-face scanning electron microscopy, with 36 of the complete set of 46 chromosomes captured within it.
Inter-chromosomal transcription hubs shape the 3D genome
6 天之前 · Specifically, the rDNA loci on chromosomes 1, 3, and 7 form one cluster, while two rDNA genes on the subtelomeric region of chromosome 11 A interact with the rRNA clusters on chromosomes 9 and 10 ...
Review Chromosome positioning in the interphase nucleus
2002年9月1日 · Metaphase chromosomes display a chromosome-specific series of alternating light- and dark-staining bands (R- and G-bands), corresponding to early- and late-replicating regions of the genome. During interphase, these regions are maintained and occupy distinct domains within a chromosome territory 10. , 14. , 15. .