Use of luciferase probes to measure ATP in living cells and animals
2017年7月6日 · For intracellular ATP measurement, luciferin should be used in a concentration ranging from 20 to 200 μM, as proportionality between luminescence values and perfused luciferin is maintained...
Lighting up ATP in cells and tissues using a simple aptamer-based ...
2021年9月23日 · Study with model cell U2OS: the ATP aptamer-based probe senses intracellular ATP and facilitates live cell imaging. Having demonstrated the signaling ability of the aptamer-based ATP probe in solution, we next tested its ability to detect intracellular ATP. Oligonucleotides do not enter cell actively.
Approaches to monitor ATP levels in living cells: where do we …
2021年8月26日 · This biosensor allows the monitoring of intracellular ATP in living HeLa cells with subcellular resolution: ATP was detected in the cytoplasm, nucleus, and the mitochondria by adding an organelle-specific localization signal to the biosensor.
ATP biosensor reveals microbial energetic dynamics and ... - Nature
2024年6月21日 · Adenosine-5’-triphosphate (ATP), the primary energy currency in cellular processes, drives metabolic activities and biosynthesis. Despite its importance, understanding...
Aptamer based probes for living cell intracellular molecules …
2022年7月15日 · The application of aptamer-based fluorescent probes for intracellular molecular imaging in living cells were summarized. •. The properties and mechanisms of different fluorescent aptamer biosensors were described and illustrated. •. In situ biological imaging of disease-related biomolecules in living cells were summarized. •.
DNA tetrahedron-based split aptamer probes for reliable imaging of ATP ...
2023年3月1日 · Here, we developed a DNA tetrahedron-based split aptamer probe (TD probe) for ratiometric fluorescence imaging of ATP in living cells. The TD probe is constructed by hybridizing two split ATP aptamer probes (Apt-a and Apt-b) to a DNA tetrahedron assembled by four DNA oligonucleotides (T1, T2, T3 and T4).
Intracellular Detection of ATP Using an Aptamer Beacon …
2014年11月13日 · To address nonspecific probe displacement and the false positive signal, we herein report a covalently linked aptamer probe for adenosine triphosphate (ATP) detection. A fluorophore and amino dual modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ∼50%.
Molecule fluorescent probes for adenosine triphosphate imaging …
2022年2月1日 · Adenosine triphosphate (ATP) is the primary energy source for all forms of work in cells, which is closely associated with cancers. Detection of ATP in specific intracellular compartment is crucial to precisely understand many biological processes.
Recent advances in fluorescent probes for ATP imaging
Consequently, researchers have developed many fluorescent probes for ATP detection, recognizing the importance of monitoring intracellular ATP levels to understand cellular processes. These probes have been effectively utilized for visualizing ATP in living cells and biological samples.
Real-Time Imaging of Mitochondrial ATP Dynamics Reveals the …
2018年10月9日 · Here, we used these fluorescent probes targeted to distinct cellular compartments in order to investigate the dynamics of intracellular ATP pools in response to acute glucose removal, glucose substitution, as well as mitochondrial toxins.