双特异抗体错配防止策略--重链设计 - 知乎 - 知乎专栏
KiH是上世纪90年代Gennetech团队发明的防止 重链错配 技术，该技术对抗体的CH3进行了点突变（图1）；在一个抗体的重链（Knob链）的CH3第366位丝氨酸T突变为色氨酸W；在另外一条重链（Hole链）的第366位丝氨酸T突变为丝氨酸S，第368位亮氨酸L突变为 丙氨酸 A，第407位 ...
Knobs-into-holes antibody production in mammalian cell lines …
Knobs-into-holes is a well-validated heterodimerization technology for the third constant domain of an antibody. This technology has been used to produce a monovalent IgG for clinical development (onartuzumab) and multiple bispecific antibodies.
Roche的FORCE双抗技术：knob-into-hole升级版 - 知乎 - 知乎专栏
2021年3月22日 · Roche的FORCE双抗技术：knob-into-hole升级版双抗技术的飞速发展，产生了数十个技术平台，并基于这些技术平台产生了上百个Formats。 如何通过不同的format实现双特异性抗体的生物功能，必需考量和优化非常多的参数…
knob-into-hole（KIH）的开发 - 平头哥
2023年8月1日 · Ab为CD3抗体和IA为CD4-Fc融合蛋白，分别在CD3抗体和CD4-Fc融合蛋白的Fc区域引入knob或者hole突变，然后将突变后的序列组合表达，并通过调整不同的质粒比例以优化Ab/IA产量。
Knobs-into-holes antibody production in mammalian cell lines …
Knobs-into-holes is a well-validated heterodimerization technology for the third constant domain of an antibody. This technology has been used to produce a monovalent IgG for clinical development (onartuzumab) and multiple bispecific antibodies. 1,2 The most advanced uses of this approach, however, have been limited to E. coli as an expression ...
Production of bispecific antibodies in "knobs-into-holes" using …
Among extremely diverse architectures of bispecific antibodies, knobs-into-holes (KIHs) technology, which involves engineering CH3 domains to create either a "knob" or a "hole" in each heavy chain to promote heterodimerization, has been widely applied.
Probing the Impact of the Knob-into-Hole Mutations on the …
2019年12月9日 · The knob-into-hole approach is an effective way to produce bispecific antibodies by driving heterodimerization with mutations in the CH3 domain of each half antibody.
Overview of Knobs-in-holes Bispecific Antibody - Creative Biolabs
Knobs-in-holes (KIHs) bispecific antibody is a type of bispecific IgG antibody that has a heterodimeric Fc region and two different Fab arms. The Fc region is composed of two distinct heavy chains that are engineered to form a stable and specific heterodimer by introducing a "knob" and a "hole" mutation in the CH3 domains.
Full article: Knobs-into-holes antibody production in mammalian …
2013年8月29日 · Knobs-into-holes technology has become the foundation for a well-developed E. coli bispecific antibody production process, offering biochemical qualities comparable to conventional monoclonal antibodies and scalable manufacture compatible …
‘Knobs-into-holes’ engineering of antibody C - Oxford Academic
1996年7月1日 · ‘Knobs-into-holes’ is demonstrated here as a novel and effective design strategy for engineering antibody heavy chain homodimers for heterodimerization. In this approach a ‘knob’ variant was first obtained by replacement of a small amino acid with a larger one in the C H 3 domain of a CD4-IgG immuno-adhesin: T366Y.