Rat brain endothelial cell lines for the study of blood-brain barrier ...
WEBThe present review will constitute a brief description of the best characterized RBE cell lines (RBE4, GP8/3.9, GPNT, RBEC1, TR-BBBs and rBCEC4 cell lines) and will summarize their recent and important contribution to our current knowledge of the BBB transport functions and permeability to blood-borne solutes, drugs, and cells.
Cellosaurus cell line RBE4 (CVCL_0495)
WEBEvaluation of the RBE4 cell line to explore carrier-mediated drug delivery to the CNS via the L-system amino acid transporter at the blood-brain barrier. J. Drug Target. 10:277-283(2002) PubMed= 12958429 ; DOI= 10.1385/1-59259-419-0:307
Cx43 channels and signaling via IP - Nature
WEBMar 18, 2020 · We performed live cell Ca 2+-signal imaging experiments starting 5 min after broad-beam irradiation of RBE4 cells, which demonstrated significantly increased Ca 2+ oscillations in the...
Comparison of a Rat Primary Cell-Based Blood-Brain Barrier ... - PubMed
WEBMay 22, 2018 · Cell culture-based blood-brain barrier (BBB) models are useful tools for screening of CNS drug candidates. Cell sources for BBB models include primary brain endothelial cells or immortalized brain endothelial cell lines.
Flavonoid transport across RBE4 cells: A blood-brain barrier model
WEBFeb 8, 2010 · The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport.
Comparison of a Rat Primary Cell-Based Blood-Brain Barrier …
WEBMay 22, 2018 · Among the brain endothelial cell lines, the rat GP8 (Greenwood et al., 1996) and RBE4 (Roux et al., 1994), and human hCMEC/D3 cells (Weksler et al., 2005) are the best characterized and the most widely used in BBB research.
Mitochondrial Changes in Rat Brain Endothelial Cells Associated …
WEBIn endothelial cell line (RBE4) after 24 h ammonia and/or TNFα treatment, conditions mimicking crucial aspects of HE in vivo, we observed altered expression of mitochondrial fission/fusion genes: a decrease of opa1, mfn1, and, increase of the fission related fis1 gene. The effect in vitro was paralleled by the generation of reactive oxygen ...
RBE4 cells are highly resistant to paraquat-induced ... - PubMed
WEBRBE4 cells were shown to be extremely resistant to PQ after 24 h of exposure; even at concentrations as high as 50 mM approximately 45% of the cells remained viable. Prolonging exposure until 48 h elicited significant cytotoxicity only …
Developmental Aspects of Blood–Brain Barrier (BBB) and Rat Brain ...
WEBAug 1, 2003 · Among different cell lines, the immortalized RBE4 cell line has shown great promise as an in vitro BBB model (Rist et al., 1997). RBE4 cells were derived from rat brain microvascular endothelium that was immortalized with the plasmid pE1A-neo, which contains the E1A region of adenovirus 2 along with …
Developmental Aspects of Blood–Brain Barrier (BBB) and Rat Brain ...
WEBAug 1, 2003 · In this study we assessed tight junction disruption at the protein and gene expression levels using a rat brain microvascular endothelial cell line (RBE4) exposed to lead acetate at 10 −5 M and 10 −6 M, malathion at …
- Some results have been removed