Single-cell sequencing - Wikipedia
Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular …
Single-cell sequencing techniques from individual to multiomics ...
2020年9月15日 · Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and …
Understanding Single-Cell Sequencing, How It Works and Its …
2024年2月26日 · What is single-cell sequencing and what is single-cell RNA sequencing? Traditional next-generation sequencing (NGS) examines the genome of a cell population, such …
Single‐cell RNA sequencing technologies and applications: A …
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as …
The technological landscape and applications of single-cell
2023年6月6日 · Single-cell multi-omics technologies and methods characterize cell states and activities by simultaneously integrating various single-modality omics methods that profile the …
Single-cell sequencing to multi-omics: technologies and …
2024年9月27日 · Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell …
Single-cell RNA sequencing technologies and applications: A …
Single-cell RNA sequencing (scRNA-seq) technology has become the state-of-the-art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as …
The single-cell sequencing: new developments and medical …
2019年6月26日 · This review introduced single-cell sequencing methods and their applications in tumors, microorganism, nervous system, reproductive medicine and immunology, highlighting …
Single-cell genomics technology: perspectives - PMC - PubMed …
Single-cell sequencing technology has also evolved over time, from processing dozens of cells to millions of cells simultaneously. New approaches to well-established models are being …
Single cell sequencing: a distinct new field - PMC
2017年2月20日 · Single cell sequencing methods. The advance in the next-generation sequencing (NGS) technologies has promoted the rapid development of SCS, including single …
Single-cell RNA sequencing technologies and bioinformatics …
2018年8月7日 · Single-cell RNA sequencing (scRNA-seq), for example, can reveal complex and rare cell populations, uncover regulatory relationships between genes, and track the …
Single-Cell Sequencing - an overview | ScienceDirect Topics
Single-cell sequencing (SCS) is a next-generation sequencing method that is mainly used to analyze differences in genetic and protein information between cells, to obtain genetic …
Single-Cell RNA Sequencing Procedures and Data Analysis
2021年3月20日 · A typical single-cell sequencing workflow involves initial tissue and cell preparation, cell capturing and library preparation, sequencing and raw data processing, as …
Single-Cell Applications of Next-Generation Sequencing - PMC
Next-generation sequencing (NGS) of RNA and DNA has opened a new frontier of (single)-cell biology. Hundreds to millions of cells now can be assayed in parallel, providing the molecular …
Single Cell Portal - Broad Institute
Single-cell sequencing has revolutionized the scale and resolution of molecular profiling of tissues and organs. Here, we present an integrated multimodal reference atlas of the most accessible …
Explore every step of the single-cell sequencing workflow and learn valuable insights to ensure experimental success. For Research Use Only. Not for use in diagnostic procedures. …
Single-Cell and Low-Input RNA-Seq | Single-cell sequencing …
Highly sensitive ultra-low-input and single-cell RNA sequencing (RNA-Seq) methods enable researchers to explore the distinct biology of individual cells in complex tissues and …
Single-Cell RNA Sequencing Reveals the Developmental …
1 天前 · Single-cell RNA sequencing (scRNA-seq) was used to unravel the transcriptome heterogeneity of cells and the composition of cell types in broad-spectrum organisms. Here, …
Single-cell sequencing techniques from individual to multiomics ...
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and …
Single-cell RNA sequencing reveals the critical role of alternative ...
2024年12月26日 · Spermatogonial stem cells (SSCs) form haploid gametes through the precisely regulated process of spermatogenesis. Within the testis, SSCs undergo self-renewal through …
Single-cell sequencing - Nature Methods
2013年12月30日 · Single-cell sequencing can enable the discovery of clonal mutations, cryptic cell types or transcriptional features that would be diluted or averaged out in bulk tissue. …
Illumina advances NovaSeq X Series, delivering single-flow-cell …
1 天前 · Now in the market for the first time, the single-flow-cell NovaSeq X Sequencing System offers the same high-quality flow cells and speed as the NovaSeq X Plus Sequencing …
Applications of Single-cell DNA Sequencing - PMC - PubMed …
Here, we review the applications and biological questions for which single-cell DNA sequencing is uniquely suited or required. We include a discussion of the fields that will be impacted by …
Illumina Single Cell 3' RNA Prep
Choose the size kit to match the number of cells you want to assay. Kits are also available separately as Illumina Single Cell 3' RNA Capture and Illumina Single Cell Library Prep. For …
Single-nucleus RNA sequencing reveals distinct …
1 天前 · Single-nucleus RNA sequencing. In an earlier study, we applied scRNA-seq to examine freshly collected chorionic villi samples for spontaneous miscarriages [].In this study, we …
A joint analysis of single cell transcriptomics and proteomics …
6 天之前 · Recent advancements in mono-omics research, particularly through single-cell RNA sequencing (scRNA-seq) methods, have already evolved to revolutionize our knowledge of …
Current best practices in single‐cell RNA‐seq analysis: a tutorial
Here, we detail the steps of a typical single‐cell RNA‐seq analysis, including pre‐processing (quality control, normalization, data correction, feature selection, and dimensionality reduction) …